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采用田口设计法对TPGS单酯与双酯的柱层析分离工艺进行优化。采用Minitab 15软件设计正交试验方案,并分析不同因素不同水平下的信噪比值。实验结果表明TPGS单酯与双酯回收率的最优方案分别为上样量1.5 g、上样浓度0.4 g/mL、流速4 mL/min、柱温30℃和上样量1.5 g、上样浓度0.3 mg/mL、流速3 mL/min、柱温30℃。田口方法预测的TPGS单酯与双酯回收率最优结果分别为95.19%和96.12%,验证实验结果分别为94.89±0.56%和95.08±0.31%。结果表明所优化方案具有较好稳定性,为工业分离提供了参考。
Taguchi design method was used to optimize the column chromatography separation process of TPGS monoester and diester. Orthogonal experimental design was designed with Minitab 15 software, and the signal-to-noise ratio under different levels of different factors was analyzed. The experimental results showed that the optimal solutions of TPGS monoester and diester recovery were as follows: sample loading 1.5 g, sample loading 0.4 g / mL, flow 4 mL / min, column temperature 30 ℃ and sample loading 1.5 g, Concentration 0.3 mg / mL, flow rate 3 mL / min, column temperature 30 ℃. Taguchi method predicted the best results of TPGS monoester and diester recovery were 95.19% and 96.12%, respectively. The validation results were 94.89 ± 0.56% and 95.08 ± 0.31%, respectively. The results show that the optimized scheme has good stability and provides a reference for industrial separation.