Affinty membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from fitterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membrane cartridge can be used for the removal of endotoxin from aqueous solutions. With a cartridge of 40 sheets affinity membrane containing,the endotoxin level of the processed solution can be reduced to a minimum of 4 pg/mL. Affinty membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from fitterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membrane cartridge can be used for the removal of endotoxin from aqueous solutions. With a cartridge of 40 sheets affinity membrane containing, the endotoxin level of the processed solution can be reduced to a minimum of 4 pg / mL.