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鸡胚绒毛尿囊膜(chicken chorioallantoic mem-brane.CAM)是进行组织在体培养的良好模型.种植于CAM的人蜕膜组织(decidual tissue.DT)具有生长分化的潜力,可由该模型上的透明窗对活体生长组织进行连续动态观察.为研究DT培养的影响因素,探讨早孕期组织血管生成的课题,对种植于CAM的DT生长过程作了进一步观察.材料和方法实验方法见文献.北京白鸡种蛋,鸡胚发育第7天制备假气室.同时种植DT.每12h自观察窗对种植组织进行观察.用目镜测微尺测定组织大小.连续观察11d.共进行四批实验,种植4例早孕人流的DT,89个鸡胚.每个鸡胚种植2mm~2左右DT4～6块.结果DT在CAM的生长过程可分为五个阶段:1.静止期 DT种植后12～15h,部分组织的中央处色泽略浅.边缘部分组织的色泽保持不变.DT小块逐渐平铺展开,与CAM的接触面增大,CAM血管
Chicken chorioallantoic mem- brane.CAM is a good model for in vivo tissue culture, and the decidual tissue .DT implanted in CAM has the potential to grow and differentiate, Transparent window on the growth of living tissue for continuous dynamic observation.In order to study the influencing factors of DT culture and to explore the issue of tissue angiogenesis in early pregnancy, DT growth process in CAM was further observed.Materials and methods of experimental methods see the literature.Beijing The white chicken eggs were cultured for 7 days, and the fake air chamber was also prepared.The DT was planted at the same time.The planting tissues were observed from the observation window every 12 hours.The size of the tissues was measured with eyepiece micrometer and observed continuously for 11 days.A total of four batches of experiments 4 cases of early pregnancy flow of DT, 89 chicken embryos.Each embryo to grow about 2mm ~ 2 DT4 ~ 6. Results DT in the CAM growth process can be divided into five stages: 1. Quiescent DT after planting 12 ~ 15h , Some organizations in the central color slightly lighter.The edge of the organization part of the color remains unchanged.DT pieces gradually tiled, with the CAM contact surface increases, CAM blood vessels