论文部分内容阅读
目的通过腺样囊性癌（adenoid cystic carcinoma，ACC）在纤维连接蛋白（fibronectin,FN）中的固相趋化功能变化探讨其浸润机制。方法用Boyden小室法和流式细胞术等测定了3种ACC细胞对FN的趋化反应和细胞表面的受体变化。结果 3种ACC均比鳞状细胞癌（squamous cell carcinoma,SCC）趋化功能强；ACC和SCC细胞表面α2、α3、α6和β1受体亚单位水平高，且ACC3α5也高；受体阻断可见：FN中阻断ACC的α5、β1受体亚单位趋化功能呈高度抑制，阻断α4呈中度抑制，SCC则阻断α1、α4和β1呈强抑制。结论 FN中ACC比SCC侵袭力强；各种细胞不同受体在FN中趋化反应的作用有一定规律。“,”Objective To investigate the invasion mechanism of adenoid cystic carcinoma(ACC) by ACC haptotaxis changes in fibronectin (FN). Methods Three strains of ACC cells were used. The haptotaxis changes in FN, and receptor changes in the cell membrane surface were determined. Results All three strains of ACC cells had remarkably greater migration ability than SCC cell. High level of α2,α3,α6 and β1 receptor subunit types was found in the cell surface of ACC and SCC; and high level of α5 in ACC3. High inhibition of haptotaxis reaction was found after blocked α5 and β1 receptor subunit in ACC and a middle inhibition after blocked α4. In SCC more strong inhibition was found after blocked α1, α4 and β1. Conclusions This study indicates that ACC cells have greater invasion ability than SCC cells in FN and haptotaxis reaction of different receptor of tested cells in FN have some feature pattern.