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目的了解中国安徽省马鞍山市分离到的枸橼酸杆菌的分子流行病学特征。方法 2007 2011年从安徽省马鞍山市的腹泻患者粪便、食品和食品加工者肛拭分离到62株枸橼酸杆菌,包括13株弗氏枸橼酸杆菌、41株杨氏枸橼酸杆菌和8株布氏枸橼酸杆菌。所有菌株用XbaⅠ酶切进行脉冲场凝胶电泳(PFGE)分型和扩增7个管家基因的多位点序列分型(MLST)分析。结果 PFGE将62株枸橼酸杆菌分成43个带型(PTs),MLST将其分成53个ST型别(STs)。CITX01.CN0039是PFGE的优势带型,由2007-2008年间从食品和腹泻患者粪便分离的7株杨氏枸橼酸杆菌组成。53个ST型别分成5个ST序列群,ST序列群39个优势菌株来自腹泻患者粪便、食品和食品加工者肛拭分离的枸橼酸杆菌。从腹泻患者粪便、食品和食品加工者肛拭分离得到的枸橼酸杆菌具有相同的PTs和STs。结论在食品和人群中监测枸橼酸杆菌对预防和控制枸橼酸杆菌引起的腹泻是必要的。
Objective To understand the molecular epidemiology of Citrobacter isolated in Ma’anshan City, Anhui Province, China. Methods In 2011, 62 citrobacter were isolated from feces, food and food processors in diarrhea patients in Ma’anshan City, Anhui Province. In 2011, 62 citrobacter were isolated from 13 strains of Citrobacter freundii, 41 strains of Citrobacter young and 8 Strains of Citrobacter. All strains were digested with XbaI and analyzed by multi-site sequence typing (MLST) using pulse field gel electrophoresis (PFGE) and amplification of seven housekeeping genes. Results PFGE divided 62 Citrobacters into 43 bands (PTs), which were divided into 53 ST types (STs) by MLST. CITX01.CN0039 is the dominant band of PFGE and consists of 7 strains of Citrobacter youngatus isolated from feces of patients with food and diarrhea during 2007-2008. Fifty-three ST genotypes were divided into five ST sequence groups, and 39 dominant strains from the ST sequence group were Citrobacter citric acid isolated from faeces, food and food processors in diarrhea patients. Citrobacter isolates from fecal, food and food processors in diarrhea patients have the same PTs and STs. Conclusion Monitoring Citrobacter in foods and people is necessary to prevent and control citrobacter-induced diarrhea.