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以前期获得的紫苏HPPR基因的c DNA序列为模板,通过设计特异性引物和染色体步移的方法分离出HPPR基因启动子,全长2 216 bp,以此研究HPPR基因启动子的结构及功能特点。生物信息学分析表明,该序列中存在TATA-box和CAAT-box等典型的真核生物启动子基本元件,以及对茉莉酸甲酯、生长素、水杨酸和脱落酸等植物激素应答相关的顺式作用元件,另外也存在非生物胁迫顺式作用元件。将HPPR启动子部分缺失序列替代p BI121载体中的Ca MV35S启动子,构建了与GUS融合的启动子元件缺失表达载体。本试验的研究为该启动子在今后紫苏转基因定向改良提供了有用的候选资源。
Based on the c DNA sequence of perilla HPPR gene obtained from the previous study, the HPPR gene promoter was isolated by designing specific primers and chromosome walking. The full-length HPPR gene promoter was 2 216 bp in length to study the structure and function of HPPR gene promoter Features. Bioinformatics analysis showed that there are typical eukaryotic promoters such as TATA-box and CAAT-box in this sequence, as well as phytohormones such as methyl jasmonate, auxin, salicylate and abscisic acid Cis-acting elements, in addition there are also abiotic stress cis-acting elements. A partial deletion sequence of HPPR promoter was substituted for CaMV35S promoter in pBI121 vector to construct a promoter deletion vector for expression with GUS. The research of this experiment provided a useful candidate resource for future improvement of transgene of Perilla frutescens.