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用活细胞内荧光探针 Ｆｕｒａ２／ＡＭ检测 ＣＮＴＦ对谷氨酸引起的海马神经细胞内游离Ｃａ２＋浓度升高的快速影响，并观察Ｇ蛋白是否参与．结果表明，谷氨酸可引起海马神经细胞内游离 Ｃａ２＋浓度快速升高，并在５００μｍｏｌ／Ｌ范围内呈剂量相关性． ＣＮＴＦ对静息条件下海马神经细胞内的游离Ｃａ２＋浓度无显著性改变．但ＣＮＴＦ孵育５ｍｉｎ后，可快速压抑谷氨酸引起的胞内游离Ｃａ２＋浓度升高，这一过程有ＰＴＸ敏感的Ｇ蛋白参与．ＣＮＴＦ这一作用时程提示，可能不经由ＣＮＴＦ的基因组信号转导途径，在上游即有Ｇ蛋白参与的快捷支路．
Fura2 / AM, a fluorescent probe of living cells, was used to detect the rapid effect of CNTF on glutamate-induced increase of intracellular free Ca2 + concentration in hippocampal neurons and to observe whether G protein is involved. The results showed that glutamate induced a rapid increase of intracellular free calcium concentration in hippocampal neurons and a dose-dependent manner in the range of 500 μmol / L. CNTF had no significant change on the concentration of free Ca2 + in hippocampal neurons resting at rest. However, CNTF incubated for 5min can rapidly depress glutamate-induced increase of intracellular free Ca2 + concentration. PTX-sensitive G protein is involved in this process. The role of CNTF as a time course suggests that there may be a shortcut arm for the participation of G proteins upstream, possibly via the genomic signal transduction pathway of CNTF.