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以细胞的存活率、回收率和三项免疫功能(淋巴细胞转化、IL—2产生能力和LAK活性)为指标,对人外周血淋巴细胞(PBL)在VARI-RATEⅢ型冷冻仪的冻存条件下进行了系统研究,找出了细胞冻存的适宜条件,即细胞在4℃与含10%的DMSO的1640培液平衡15分钟后,以-10C/分的速度降温至-30℃,然后立即放入液氮保存。冷冻细胞的浓度以5×10~6/ml～1×10~7/ml左右为宜。采用慢稀释法复苏细胞使其功能得到较好的恢复。并将冷冻PBL与同一供体的新鲜PBL进行比较,证明了冷冻PBL能真实反映冷冻前个体差异的真实情况。
The cell survival rate, recovery and three immune functions (lymphocyte transformation, IL-2 production and LAK activity) as indicators, the human peripheral blood lymphocytes (PBL) in VARI-RATE Ⅲ freezer cryogenic storage conditions A systematic study was conducted to find out the suitable conditions for cell cryopreservation. After the cell was equilibrated with 1640 culture solution containing 10% DMSO at 4 ° C for 15 minutes, the cell was cooled to -30 ° C at a rate of -10 ° C / min and then Immediately into liquid nitrogen preservation. Frozen cells concentration of 5 × 10 ~ 6 / ml ~ 1 × 10 ~ 7 / ml is appropriate. Recovery of cells by slow dilution method to make its function better recovery. And comparing the frozen PBL with the fresh PBL of the same donor, it is proved that the frozen PBL can truly reflect the real situation of individual differences before freezing.