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目的探讨胃癌组织中血管内皮生长因子C(VEGFC)的表达及与肿瘤淋巴结转移的关系。方法取30例胃癌患者手术切除的新鲜标本和距癌灶5cm以外的胃组织各1块,采用半定量RTPCR法和免疫组化染色法检测胃癌组织及癌旁正常组织的VEGFC表达,图像分析仪检测VEGFC的免疫组化阳性指数(positiveindex,PI)。结果胃癌细胞呈高水平表达VEGFC,癌旁正常组织则未见表达。在胃癌组织中,有淋巴结转移(PI=1.345±0.079)、淋巴管浸润(PI=1.315±0.037)患者的VEGFC表达水平分别较无转移(PI=1.156±0.045)及无浸润者(PI=1.154±0.043)增高(P<0.05)。高分化胃癌患者的VEGFCmRNA[VEGFC/βactin比值(Ar)=0.846±0.042]和VEGFC表达(PI=1.372±0.023)均分别高于中低分化胃癌者(Ar=0.663±0.007,P<0.01和PI=1.126±0.078,P<0.05)。结论VEGFC阳性表达的胃癌患者可能更易发生淋巴结转移。
Objective To investigate the expression of vascular endothelial growth factor C (VEGFC) and its relationship with lymph node metastasis in gastric cancer. Methods Fresh specimens of 30 patients with gastric cancer and one gastric tissue 5 cm away from the tumor were collected. The expression of VEGFC was detected by semi-quantitative RT-PCR and immunohistochemical staining in gastric cancer tissues and adjacent normal tissues. The image analyzer The positive index of VEGFC (positiveindex, PI) was detected. Results The gastric cancer cells showed a high level of expression of VEGFC, while the adjacent normal tissues showed no expression. In gastric cancer, the expression of VEGFC in patients with lymph node metastasis (PI = 1.345 ± 0.079) and lymphatic invasion (PI = 1.315 ± 0.037) were significantly higher than those without metastasis (PI = 1.156 ± 0.045) ± 0.043) (P <0.05). VEGFC mRNA [VEGFC / βactin ratio (Ar) = 0.846 ± 0.042] and VEGFC expression (PI = 1.372 ± 0.023) in patients with well-differentiated gastric cancer were significantly higher than those in patients with moderately poorly differentiated gastric cancer (Ar = 0.663 ± 0.007, P <0.01 and PI = 1.126 ± 0.078, P <0.05). Conclusion The patients with gastric cancer with positive VEGFC expression may be more likely to have lymph node metastasis.