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目的探讨一氧化氮(NO)、超氧化物歧化酶(SOD)在反流性食管炎(RE)患者发病中的作用。方法选择反流性食管炎患者50例作为 RE 组,慢性胃炎患者30例作为对照组。采用NADPH-d 酶组化染色法对 RE 和对照组食管黏膜组织中的一氧化氮合酶(NOS)进行定性检验,应用硝酸还原法和黄嘌呤氧化酶法分别测定血中及食管组织中的 NO 和 SOD 含量。结果 RE 组患者食管黏膜大多呈 NOS 阳性反应,阳性率明显高于对照组(P<0.01),RE 组患者食管黏膜组织及血清 NO含量也明显高于对照组(P<0.01),而 RE 组患者食管黏膜组织及血清的 SOD 含量明显低于对照组(P<0.01)。不同程度 RE 食管组织的 NO 含量与 SOD 含量具有一定的相关性(r=-0.645,P<0.01)。结论 NO、SOD 在 RE 患者的发病中可能共同发挥着重要的作用。
Objective To investigate the role of nitric oxide (NO) and superoxide dismutase (SOD) in the pathogenesis of reflux esophagitis (RE). Methods Fifty patients with reflux esophagitis were selected as RE group and 30 patients with chronic gastritis as control group. The nitric oxide synthase (NOS) in esophageal mucosa of RE and control group was qualitatively tested by NADPH-d enzymatic histochemical staining. Nitric acid reduction and xanthine oxidase method were used to determine the levels of NO and SOD content. Results Most of esophageal mucosa of RE group showed positive NOS positive reaction, the positive rate was significantly higher than that of control group (P <0.01). The content of NO in esophageal mucosa tissue and serum in RE group was also significantly higher than that in control group (P <0.01) Patients with esophageal mucosa and serum SOD levels were significantly lower than the control group (P <0.01). There was a correlation between NO content and SOD content in esophageal tissues (r = -0.645, P <0.01). Conclusion NO and SOD may play an important role in the pathogenesis of RE.