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目的探讨妊娠期糖尿病(GDM)妇女孕晚期脂肪细胞胰岛素受体底物-1(IRS-1)表达的改变。方法健康孕妇和GDM孕妇各20例,入院第2天采用葡萄糖氧化酶法测定空腹血糖和餐后2h血糖,剖宫产时取皮下脂肪约1cm3,用Wester-blot方法测定IRS-1。结果GDM孕妇空腹血糖与健康孕妇差异无统计学意义(P>0.05);GDM孕妇餐后2h血糖较健康孕妇显著升高(P<0.05);GDM孕妇脂肪细胞IRS-1含量显著低于健康孕妇(P<0.05)。结论GDM孕妇与健康孕妇相比皮下脂肪细胞IRS-1表达显著降低。
Objective To investigate the changes of insulin receptor substrate-1 (IRS-1) in third trimester of pregnancy in gestational diabetes mellitus (GDM). Methods 20 healthy pregnant women and 20 pregnant women with GDM were enrolled. On the second day after admission, the fasting blood glucose and postprandial blood glucose were determined by glucose oxidase method. The subcutaneous fat was about 1cm3 at cesarean section. IRS-1 was determined by Wester-blot method. Results There was no significant difference between fasting blood glucose and healthy pregnant women in GDM (P> 0.05). The blood glucose of GDM pregnant women was significantly higher than that of healthy pregnant women at 2h (P <0.05); the content of IRS-1 in GDM pregnant women was significantly lower than that of healthy pregnant women (P <0.05). Conclusion The expression of IRS-1 in subcutaneous adipocytes of GDM pregnant women is significantly lower than that of healthy pregnant women.