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目的:研究蝶豆根叶治疗糖尿病的功效,对影响神经系统的化学物质的抗氧化作用,以及缓解因糖尿病引起的认知减退的作用。方法:通过测定实验大鼠血清葡萄糖浓度和体质量研究蝶豆根乙醇提取物的抗糖尿病活性。通过Y字形迷宫以及莫里斯水迷宫测试分别评估蝶豆根对实验大鼠空间记忆以及空间参考记忆的影响;其对影响神经系统的化学物质的抗氧化作用则通过乙酰胆碱酯酶实验以及糖尿病模型大鼠的脂类过氧化物、超氧化物歧化酶和过氧化氢酶水平来测定。结果:服用200和400mg/kg的蝶豆根提取物后,糖尿病模型大鼠血清葡萄糖水平明显降低(P<0.01),且服用400mg/kg蝶豆根提取物的糖尿病模型大鼠的体质量有明显增加(P<0.01)。蝶豆跟提取物可以显著改善实验大鼠的空间记忆以及空间参考记忆(P<0.05,P<0.01)。与糖尿病模型对照组相比,服用200和400mg/kg蝶豆根提取物的糖尿病模型大鼠,其乙酰胆碱酯酶、脂类过氧化物显著减少(P<0.05),过氧化氢酶水平显著增加(P<0.05或P<0.01);服用400mg/kg蝶豆根提取物的糖尿病大鼠的超氧化物歧化酶水平增加(P<0.01)。结论:蝶豆根具有抗糖尿病、抗氧化的作用,并可改善由糖尿病引起的认知减退,其机制还需要进一步的研究。
OBJECTIVE: To study the efficacy of butterfly bean root and leaf in the treatment of diabetes mellitus, the antioxidant effect on the chemical substances that affect the nervous system, and the alleviation of the cognitive decline caused by diabetes mellitus. Methods: The antidiabetic activity of ethanol extract of Artemisia laevigata was studied by measuring serum glucose concentration and body weight in experimental rats. Through the Y-shaped maze and Morris water maze test, respectively, to assess the spatial and spatial memory of the test rats, and its impact on the antioxidant effect of the chemical substances of the nervous system by acetylcholinesterase test and diabetes model Rat lipid peroxides, superoxide dismutase and catalase levels. Results: After administration of 200 and 400 mg / kg of Beaulieu root extract, the serum glucose level was significantly decreased in diabetic rats (P <0.01). The body weight of diabetic rats fed with 400 mg / Significantly increased (P <0.01). Butterflies and extracts can significantly improve spatial memory and space reference memory in experimental rats (P <0.05, P <0.01). Compared with the diabetic model control group, the diabetic model rats that took 200 and 400 mg / kg of Artemisia scoparia had a significant decrease in acetylcholinesterase and lipid peroxidation (P <0.05) and a significant increase in catalase (P <0.05 or P <0.01). The level of superoxide dismutase in diabetic rats fed with 400 mg / kg of Artemisia sphaerocephala increased (P <0.01). CONCLUSION: Spatholobus root has anti-diabetic and anti-oxidant effects and can improve the cognitive decline caused by diabetes. The mechanism needs further study.