The capability of decolorization for commercial dyes byCoriolus versicolor fermentation broth containing laccase with orwithout immobilized mycelium was evaluated. With cell-freefermentation broth containing laccase, high decolorization ratiowas achieved for acid orange 7, but not for the other dyesconcerned. The immobilized mycelium was proved to be more efficientthan the cell-free system. All the four dyestuffs studied werefound being decolourized with certain extent by immobilizedmycelium. The repeated-batch decolorization was carried out withsatisfactory results. The experimental data showed that thecontinuous decolorization of wastewater from a printing and dyeingindustry was possible by using the self-immobilized C. Versicolor.