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目的 :了解新疆、辽宁地区HIV 1感染者细胞因子特征及其与病程的关系。方法 :采用流式细胞仪测定CD4 + T淋巴细胞数 ,34例感染者按 1993年美国CDC艾滋病监测病例分类及扩大的诊断标准分A、B两组 ,ELISA方法检测血浆细胞因子IL 12、IFN γ、IL 10、IL 6水平 ,载量仪测定HIV 1感染者体内病毒载量 ,同时检测 16例健康对照者的血浆细胞因子。结果 :①与健康对照相比 ,HIV 1感染者血浆IL 12水平显著降低 (P <0 0 1) ,IFN γ、IL 10水平显著升高 (P <0 0 1) ,IL 6水平明显升高 (P <0 0 5 )。②A组血浆IFN γ水平明显高于B组 ,IFN γ水平降低与疾病进展密切相关 (P <0 0 5 )。结论 :①新疆、辽宁地区HIV 1感染者血浆细胞因子IL 12、IFN γ、IL 10、IL 6出现异常变化 ;②HIV 1感染者的IFN γ水平可辅助判断机体细胞免疫水平预测疾病进展。
Objective: To understand the characteristics of cytokines in HIV-1 infected patients in Xinjiang and Liaoning Province and their relationship with the course of disease. Methods: The number of CD4 + T lymphocytes was determined by flow cytometry. According to the classification and expanded diagnostic criteria of CDC AIDS cases in USA in 1993, 34 cases were divided into A and B groups. The levels of IL-12, IFN- γ, IL-10 and IL-6. The viral load in HIV-1 infected patients was measured by the instrument of load and the plasma cytokines in 16 healthy controls were detected. Results: Compared with healthy controls, plasma IL-12 levels in HIV-1 infected patients were significantly decreased (P <0.01), IFNγ and IL-10 levels were significantly increased (P <0.01), IL 6 levels were significantly higher (P <0 05). ② The level of plasma IFNγ in group A was significantly higher than that in group B, and the decrease of IFNγ level was closely related to the progression of the disease (P <0.05). CONCLUSION: ① The abnormal changes of plasma cytokines IL 12, IFN γ, IL 10 and IL 6 in HIV-1 infected people in Xinjiang and Liaoning Provinces; ② IFN γ levels in HIV-1 infected patients may be helpful to predict the cellular immunity and predict the disease progression.