论文部分内容阅读
采用mRNA差异显示技术,分析水稻稻瘟病抗源材料“地谷”叶片受稻瘟病菌侵染前后的基因的表达差异,获得87个差异片段。对这87个差异片段进行了回收、重扩增与克隆,并对其中的81个片段进行了杂交鉴定。斑点杂交结果证实其中6个片段受稻瘟病菌诱导表达。进一步克隆测序并进行数据库比对分析表明其中一个与水稻4号染色体中一推测的苹果酸合成酶高度同源,一个与水稻11号染色体上的RPR1基因高度同源,RPR1基因具有保守的NBSLRR结构,并与水稻防卫反应的信号传导有关;另一个与水稻第6号染色体上一推测的硫氧还蛋白高度同源,其余3个为新的cDNA片段。
MRNA differential display technique was used to analyze the difference of gene expression before and after inoculated with Magnaporthe grisea in the rice blast resistance material “Diya”, and 87 differential fragments were obtained. The 87 differential fragments were recovered, re-amplified and cloned, and 81 of them were identified by hybridization. Dot blot results confirmed that six of them were induced by Magnaporthe grisea. Further cloning and sequencing and database comparison showed that one of them was highly homologous to a putative malate synthase in rice chromosome 4, one highly homologous to the RPR1 gene on rice chromosome 11, and the RPR1 gene had a conserved NBSLRR structure , Which is related to the signal transduction of rice defense response. The other is highly homologous to a putative thioredoxin on rice chromosome 6, and the other three are new cDNA fragments.