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目的:建立HPLC-MS/MS法测定气滞胃痛片中芍药苷的含量。方法:选用Agilent 20RBA×SB-C18反相柱(2.1 mm×30 mm,3.5μm)为色谱柱,流动相为甲醇-5 mmoL/L甲酸铵(75∶25),流速:0.4 mL/min,柱温:25℃。质谱条件:电喷雾离子源(ESI),检测方式为多离子反应监测(MRM),负离子模式,用于定量分析的离子为m/z 525~449。结果:芍药苷的线性范围为10~200μg/mL,r=0.9999;样品平均回收率为99.45%,RSD为0.75%。结论:该方法操作简便、快速,准确度好,能有效控制气滞胃痛片的质量。
OBJECTIVE: To establish a HPLC-MS / MS method for the determination of paeoniflorin in Qi and Stomach. METHODS: The mobile phase consisted of methanol-5 mmoL / L ammonium formate (75:25) at a flow rate of 0.4 mL / min using an Agilent 20 RBA × SB-C18 reverse phase column (2.1 mm × 30 mm, Column temperature: 25 ℃. MS conditions: electrospray ionization source (ESI), detection mode is multi-ion reaction monitoring (MRM), negative ion mode, the ion used for quantitative analysis is m / z 525-449. Results: The linear range of paeoniflorin was 10 ~ 200μg / mL, r = 0.9999. The average recovery was 99.45% and the RSD was 0.75%. Conclusion: The method is simple, rapid and accurate. It can effectively control the quality of qi stagnation stomach pain tablets.