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以平邑甜茶实生苗为试材,采用~(15)N同位素示踪法、气压过程分离法(BaPS)和磷酸甘油双层海绵通气法,研究酰胺态有机氮[CO(NH_2)_2-N]、铵态氮(NH_4~+-N)及硝态氮(NO_3~--N)对平邑甜茶~(15)N利用、分配和损失的影响。结果表明:5月17日施无机氮肥对植株生物量积累效果显著,铵态氮和硝态氮~(15)N利用率分别为13.68%和13.25%,显著高于有机氮肥的5.25%;早施氮肥还可有效抑制氮素的土壤损失,其中施NO_3~--N处理植株的氨挥发损失仅占施氮量的1.83%;晚施(7月15日)处理中,无机氮肥利用率较早期施用显著减少时对酰胺态有机氮利用率和氨挥发损失影响不大。所以早期施用NH_4~+-N或NO_3~--N是确保植株生长量和氮肥利用率的有效措施,后期合理配施酰胺态有机氮则是减少氮肥损失的有效途径。
In this study, the seedlings of the Eupatorium tricornutum were used as experimental materials, and the amido organic nitrogen [CO (NH_2) _2-N was studied by ~ (15) N isotope tracing method, pneumatic process separation method ], Ammonium nitrogen (NH 4 + -N) and nitrate nitrogen (NO 3 - -N) on the utilization, distribution and loss of. The results showed that the application of inorganic nitrogen fertilizers on May 17 had significant effects on the accumulation of plant biomass. The utilization rates of ammonium nitrogen and nitrate nitrogen (15) N were 13.68% and 13.25%, respectively, significantly higher than 5.25% of organic nitrogen fertilizers. Nitrogen fertilizer could also effectively restrain the soil loss of nitrogen. The ammonia volatilization loss of NO_3 ~ - N treatment plant only accounted for 1.83% of the nitrogen application rate. In the treatment of late application (July 15), the utilization rate of inorganic nitrogen fertilizer was Significant reduction in early application of amido organic nitrogen utilization and ammonia volatilization had little effect. Therefore, the early application of NH_4 ~ + -N or NO_3 ~ - N is an effective measure to ensure plant growth and nitrogen use efficiency, and the reasonable allocation of amido organic nitrogen at later stage is an effective way to reduce nitrogen loss.