用放射性同位素标记反义核酸,可方便地对反义核酸的体内行为进行研究,或者进行反义显像和反义治疗。目前已有多种不同性质的放射性同位素被用于反义核酸标记,标记方法也不断得到完善。本文对常用于反义核酸标记的放射性同位素的特点及其标记方法进行综述,介绍其在反义治疗药物和反义显像研究中的应用情况,并评述了反义核酸同位素标记存在的问题和发展趋势。 Antisense nucleic acids can be labeled with radioisotopes to facilitate the study of the in vivo behavior of antisense nucleic acids, antisense imaging and antisense therapy. At present, many different types of radioactive isotopes have been used for antisense nucleic acid labeling, and the labeling method has been continuously improved. This review summarizes the characteristics and labeling methods of radioisotopes commonly used in antisense nucleic acids and introduces their applications in the research of antisense and antisense imaging. The problems of antisense nucleic acid isotope labeling development trend.