目的:丹参根甲醇提取物体外抑菌活性评价。方法:采用牛津杯法和96孔板法,通过测定抑菌圈大小、最小抑菌浓度(MIC)和最小杀菌浓度(MBC),比较丹参根甲醇提取物对10种常见细菌的抑制作用。结果:丹参根甲醇提取物对10种常见的细菌均表现出较强的抑菌活性,表皮葡萄球菌对提取物最敏感,其MIC和MBC均为1.52mg/mL。结论:丹参提取物作为天然的抗菌物质,具有广谱抗菌效果,有较高的应用潜力和开发前景。 OBJECTIVE: To evaluate the antibacterial activity of methanol extract of Salvia miltiorrhiza in vitro. Methods: The inhibitory effect of Salvia miltiorrhiza root methanol extract on 10 common bacteria was determined by the method of Oxford cup and 96-well plate method by measuring the size of inhibition zone, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Results: methanol extract of Salvia miltiorrhiza showed strong antibacterial activity against 10 kinds of common bacteria. Staphylococcus epidermidis was the most sensitive to the extract with the MIC and MBC of 1.52 mg / mL. Conclusion: Salvia extract as a natural antimicrobial substance, with broad-spectrum antibacterial effect, has high potential and development prospects.