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目的:评价磁共振动态磷谱技术对评估2型糖尿病患者子女骨骼肌能量代谢和线粒体功能的价值。方法:对21名2型糖尿病患者子女和18名健康志愿者的股四头肌进行磁共振动态磷谱检查,获取静息、运动、恢复3个状态的波谱数据。对无机磷(Pi)、磷酸肌酸(PCr)、三磷酸腺苷(ATP)等化合物的峰下面积进行定量分析,通过生化反应平衡公式计算二磷酸腺苷(ADP)和细胞内pH值,并利用指数曲线拟合分析Pi、PCr和ADP的时间常数和恢复速率。结果:糖尿病患者子女组及正常对照组静息期Pi、PCr、ADP、β-ATP的含量及Pi/PCr无统计学差异。运动末期糖尿病患者子女组β-ATP低于正常对照组。恢复期糖尿病患者子女组多帧波谱PCr、β-ATP、pH均低于正常对照组,第3帧Pi/PCr比值比对照组高。恢复期糖尿病患者子女组PCr恢复速率明显低于正常对照组PCr恢复速率。结论:磁共振动态磷谱技术可以无创性检测2型糖尿病患者子女的能量代谢受损及线粒体功能状态。
OBJECTIVE: To evaluate the value of dynamic dynamic phosphorus spectroscopy in assessment of skeletal muscle energy metabolism and mitochondrial function in children with type 2 diabetes mellitus. Methods: The quadriceps femoris of 21 children with type 2 diabetes mellitus and 18 healthy volunteers were examined by dynamic magnetic resonance spectroscopy and the spectral data of rest, exercise and recovery were obtained. The area under the peak of inorganic phosphorus (Pi), creatine phosphate (PCr) and adenosine triphosphate (ATP) were quantitatively analyzed. The ADP and intracellular pH values were calculated by the biochemical reaction equilibrium formula. Curve fitting analyzes the time constants and recovery rates of Pi, PCr and ADP. Results: There was no significant difference in Pi, PCr, ADP and β-ATP levels and Pi / PCr between the diabetic group and the normal control group. Beta-ATP in children with end-stage diabetes was lower than that in controls. The PCr, β-ATP and pH of children in convalescent diabetic group were lower than that of normal control group, and the Pi / PCr ratio of the third frame was higher than that of control group. PCr recovery rate in children with convalescent diabetes was significantly lower than that in normal control group. Conclusion: Dynamic magnetic resonance spectroscopy can detect noninvasive detection of energy metabolism and mitochondrial function in children with type 2 diabetes mellitus.