论文部分内容阅读
目的 :观察 30例肺癌组织及癌旁肺组织MDR1 mRNA及MRP mRNA的表达。方法 :RT PCR方法。结果 :MDR1 mRNA在肺癌组织及癌旁肺组织的表达阳性率分别为 40 0 %及 16 6 7% (P =0 0 45 ) ,MDR1 mRNA的表达与细胞分化程度、临床分期及病理类型无关 ;MRP mRNA在肺癌组织及癌旁肺组织的表达分别为43 33%及 2 6 6 7% ,MRP mRNA的表达与细胞分化程度有关 ,低分化者MRP的表达明显高于中高分化者 ,P =0 0 3。腺癌的表达 (6 2 5 % )高于鳞癌 (2 1 43% ) ,P =0 0 5 8。MRP mRNA的表达与临床分期无关 ,P =0 5 11。 30例肺癌组织中MDR1 mRNA与MRP mRNA共同表达者 8例 (2 6 6 7% ) ,两者均无表达者 13例 (43 33% ) ,两者的一致性达 70 % ,仅表达MRP mRNA者 5例 ,仅表达MDR1 mRNA者 4例。结论 :MDR1 mRNA及MRP mRNA在肺癌的耐药中占有重要作用 ,两者在肺癌表达的一致性达 70 %。
Objective : To observe the expression of MDR1 mRNA and MRP mRNA in 30 lung cancer tissues and adjacent lung tissues. Method: RT PCR method. Results: The positive rates of MDR1 mRNA expression in lung cancer tissues and para-tumor lung tissues were 40% and 166.7% respectively (P=0.045). The expression of MDR1 mRNA was not related to cell differentiation, clinical stage and pathological type. The expression of MRP mRNA in lung cancer tissues and para-tumor lung tissues were 43 33% and 266.7% respectively. The expression of MRP mRNA was related to the degree of cell differentiation. The expression of MRP in poorly differentiated group was significantly higher than that in moderately well-differentiated group. P =0 0 3. The expression of adenocarcinoma (62.5%) was higher than that of squamous cell carcinoma (21.143%), P=0.058. The expression of MRP mRNA was not associated with clinical stage, P =0. In 30 lung cancers, 8 cases (26.7%) of MDR1 mRNA and MRP mRNA were co-expressed. There was no expression in 13 cases (43 33%). The agreement between them was 70%, and MRP mRNA was only expressed. In 5 patients, only 4 cases expressed MDR1 mRNA. Conclusion :MDR1 mRNA and MRP mRNA play an important role in the resistance of lung cancer. The consistency of the expression of MDR1 mRNA and MRP mRNA in lung cancer is up to 70%.