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目的:将经成骨诱导后的Wistar大鼠骨髓来源的间充质干细胞与牛脱细胞骨基质复合,构建组织工程骨,与天然骨在形态结构和功能上相似。实验着重观察不同预湿方法对构建的组织工程骨的过程-细胞与脱细胞骨基质复合和生长的影响,为有效快速构建组织工程化骨奠定理论基础。方法:实验
OBJECTIVE: To composite bone mesenchymal stem cells derived from bone marrow-derived Wistar rats with bovine acellular bone matrix to construct tissue engineered bone which is similar in morphology and function to native bone. The experiment focused on the effect of different pre-wetting methods on the constructed tissue engineering bone - the cell and the acellular bone matrix composite and growth, laid the theoretical foundation for the effective and rapid construction of tissue-engineered bone. Method: experiment