论文部分内容阅读
用过碘酸使酒石酸氧化断裂为乙醛酸,然后与对硝基苯肼縮合.生成的乙醛酸对硝基苯腙經提取純化后,用氫氧化鈉显色,建立了生物样品中酒石酸的新比色测定法.应用光电比色計时,检出限度可达5微克.光密度讀数的平均相对偏差为4±3％.生物样品测定的回收率兔尿为106±15％,狗血浆为74±6％。大量銻的存在对测定可无干扰,因此本法适用于酒石酸銻鉀分子中酒石酸部分的代謝研究.
The tartaric acid was oxidatively cleaved to glyoxylic acid with periodic acid, and then condensed with p-nitrophenylhydrazone. The glyoxylic acid produced by p-nitrophenylhydrazone was extracted and purified, and then developed with sodium hydroxide to establish tartaric acid in biological samples. The new colorimetric assay. The use of photoelectric colorimetric timing, the detection limit of up to 5 micrograms. The average relative deviation of optical density readings is 4 ± 3%. The recovery of biological samples was measured as 106 ± 15% of rabbit urine. Dog plasma was 74±6%. The presence of a large amount of deuterium may not interfere with the determination, so this method is applicable to the metabolism of tartaric acid in the potassium cerium tartrate molecule.