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目的以我国流行的周期型马来丝虫为研究对象，研究宿主体内清除虫体、减轻虫体负荷、控制丝虫感染的免疫效应机制。方法提取纯净的周期型马来丝虫微丝蚴（ｍｆ）装入微孔实验盒，然后植入 ＳＤ大鼠体内，在不同时间观察机体免疫系统对ｍｆ的杀伤作用。结果含ｍｆ的３．０μｍ微孔盒内，迁入的细胞量随植入时间延长而逐渐增多。迁入的细胞种类及百分率，各实验组之间无明显差异。免疫血清组ｍｆ被效应细胞粘附、杀伤率均明显高于正常血清组。免疫血清用抗ＩｇＧ免疫球蛋白处理后，粘附细胞虫体及虫体死亡率都大大下降。 ３．０μｍ微孔盒内大量效应细胞粘附于虫体表面。扫描电镜下，观察到效应细胞粘附的虫体表面出现坏死、剥脱。结论ｍｆ对宿主效应细胞有一定的趋化作用。宿主体内存在着抗体介导的细胞毒作用，参与细胞毒作用的抗体主要是ＩｇＧ。其主要的效应细胞是中性粒细胞、巨噬细胞和嗜酸粒细胞。
OBJECTIVE: To study the immune mechanism of filarial worm infection by studying the cyclosporidium malayi in China. Methods Purified Cyclophilus malaiian microfilariae (mf) were loaded into a micro-porous experimental box and then implanted into SD rats to observe the killing effect of the immune system on mf at different times. Results mf 3.0μm microporous cells, the amount of immigrated cells gradually increased with the implantation time. There were no significant differences between the experimental groups in the kind and percentage of cells that were moved in. Immune serum group mf effector cell adhesion, killing rate was significantly higher than the normal serum group. After the immune serum was treated with anti-IgG immunoglobulin, the mortality of adhering cells and parasites dropped significantly. A large number of effector cells in the 3.0μm microporous cell adhere to the surface of the worms. Under the scanning electron microscope, necrosis and exfoliation were observed on the surface of the parasites that adhered to effector cells. Conclusion mf has some chemotactic effect on host effector cells. Antibody-mediated cytotoxicity exists in the host, and the major antibody involved in cytotoxicity is IgG. The main effector cells are neutrophils, macrophages and eosinophils.