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目的 了解人乳头瘤病毒 (HPV)与大肠癌的发生及病理组织学的关系。方法 用多重引物PCR方法检测了 46例大肠癌标本和 36例对照组织的HPVDNA ,并与病理组织学结果进行比较。结果 大肠癌总HPV阳性率为 41.3% ,其中HPV16为 2 3 .9% ,与对照组比较 ,差异有显著性 (P <0 .0 5 )。总HPV和HPV16的阳性率 ,高分化腺癌组为 38.9%和 16 .7% ,中分化组为 38.5 %和 2 3 .1% ,低分化组为 46 .7%和 33 .3 % ,差异无显著性 (P >0 .0 5 ) ,HPV6 / 11及HPV18型阳性率各组比较 ,差异均无显著性 (P >0 .0 5 )。总HPV阳性率 ,印戒细胞癌组 (5 0 % )略高于管状腺癌、黏液腺癌和乳头状腺癌组 (P >0 .0 5 ) ;HPV6 / 11阳性仅见于管状腺癌组 (12 .5 % ,P >0 .0 5 )。HPV16型感染则存在于各型癌细胞中 (均超过 2 0 % ) ,恶性程度较高的黏液腺癌和印戒细胞癌中阳性率分别达 33 .3 %和 5 0 % ,其中印戒细胞癌显著高于管状腺癌和乳头状腺癌 (P <0 .0 5 )。结论 HPV感染 ,尤其是HPV16 ,与大肠癌有较密切的关系 ;其中HPV与癌组织细胞分类可能有一定的相关性 ,与癌细胞分化程度无明显相关性
Objective To understand the relationship between human papillomavirus (HPV) and colorectal carcinogenesis and histopathology. Methods Polymerase chain reaction (PCR) was used to detect the HPVDNA in 46 specimens of colorectal cancer and 36 controls, and compared with histopathological results. Results The positive rate of total HPV in colorectal cancer was 41.3%, of which HPV16 was 23.9%, which was significantly different from that of the control group (P <0.05). The positive rates of total HPV and HPV16 were 38.9% and 16.7% in the well-differentiated adenocarcinoma group, 38.5% and 23.1% in the moderately differentiated adenocarcinoma group, and 46.7% and 33.3% in the poorly differentiated adenocarcinoma group, respectively No significant (P> 0.05), HPV6 / 11 and HPV18 positive rate in each group comparison, the difference was not significant (P> 0.05). The positive rate of total HPV was significantly higher in signet ring cell carcinoma (50%) than in tubular adenocarcinoma, mucinous adenocarcinoma and papillary adenocarcinoma (P> 0.05). HPV6 / 11 was only found in tubular adenocarcinoma (12.5%, P> 0.05). HPV16 infection was found in all kinds of cancer cells (all more than 20%), and the positive rates of mucinous adenocarcinoma and signet-ring cell carcinoma were 33.3% and 50% respectively, of which the signet ring cells Cancer was significantly higher than tubular adenocarcinoma and papillary adenocarcinoma (P <0.05). Conclusion HPV infection, especially HPV16, has a close relationship with colorectal cancer. There may be some correlation between HPV and cancer cell classification, and has no significant correlation with the degree of cancer cell differentiation