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采用原子力显微镜与倒置显微镜在细胞层次上观察了人外周单个核细胞(PBMCs)与同种异源脐带间充质干细胞(hUC-MSCs)共培养的过程,并在单细胞水平上分析了共培养前后人外周单个核细胞的形貌和生物物理性质。结果发现:共培养后贴壁人外周单个核细胞的形态发生了很大的改变,并且表面分布着大小不一的颗粒状聚合物。利用AFM高空间分辨的力位移曲线测量系统,发现共培养72h后培养上清中人外周单个核细胞、贴壁的人外周单个核细胞的粘滞力分别是单纯培养72h的人外周单个核细胞的2倍、5倍,而细胞的硬度分别是单纯培养人外周单个核细胞的1.5倍、2倍。CCK-8检测提示,共培养过程中,干细胞的生长与外周血单个核细胞的生长出现了竞争作用。通过AFM探测人外周单个核细胞与脐带间充质干细胞共培养的可视化数据,有助于更好地了解间充质干细胞与外周血单个核细胞的相互作用。
At the cellular level, the co-culture of human peripheral mononuclear cells (PBMCs) with allogeneic umbilical cord mesenchymal stem cells (hUC-MSCs) was observed by atomic force microscopy and inverted microscope, and co-culture at the single-cell level Morphology and Biophysical Properties of Peripheral Mononuclear Cells from Pre and Post Human. The results showed that the morphological changes of adherent human peripheral mononuclear cells changed greatly after co-culture, and different sizes of granular polymers were distributed on the surface. Using AFM high spatial resolution force displacement curve measurement system, found that 72 h after co-culture supernatant of human peripheral mononuclear cells, adherent human peripheral mononuclear cells were 72 h cultured human peripheral mononuclear cells 2-fold, 5-fold, while the cell hardness were cultured in human peripheral mononuclear cells 1.5 times, 2 times. CCK-8 test suggested that during the co-culture, the growth of stem cells and peripheral blood mononuclear cells appeared competition. Using AFM to visualize the visualization of human peripheral mononuclear cells co-cultured with umbilical cord mesenchymal stem cells helps to better understand the interaction of mesenchymal stem cells with peripheral blood mononuclear cells.