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目的探讨大鼠骨髓间充质干细胞(rMSCs)移植治疗新生鼠缺氧缺血性脑损伤(HIBD)的最佳时间点。方法体外培养rMSCs传代至4～5代,同时建立新生鼠HIBD模型,分别在HIBD后2、24、72、144h进行腹腔注射移植,5周后行水迷宫行为学检测。结果水迷宫实验中,HIBD后2h移植组(A组)、HIBD后24h移植组(B组)、HIBD后72h移植组(C组)、HIBD后144h移植组(D组)、假手术组(E组)、HIBDPBS对照组(F组)逃避潜伏时间组间比较,E组及各移植组逃避潜伏时间均明显低于F组(P<0.05),A组低于B、C、D组(P<0.05),D组低于B组、C组(P<0.05)。各组平台象限泳距占总泳距的比例组间比较,E组及各移植组均明显高于F组(P<0.05),D组低于E组(P<0.05)、高于B组和C组(P<0.05)。结论 rMSCs移植可明显改善HIBD大鼠的空间学习记忆功能,在HIBD后2h进行移植效果较其他时间点好。
Objective To investigate the optimal time of transplantation of rat bone marrow mesenchymal stem cells (rMSCs) for neonatal hypoxic-ischemic brain damage (HIBD). Methods The rMSCs were passaged in vitro for 4 to 5 generations. At the same time, newborn HIBD model was established. The rats were injected intraperitoneally at 2, 24, 72 and 144 h after HIBD, respectively. The water maze behavior test was performed 5 weeks later. Results In the water maze test, the HIBD group at 2 hours after transplantation (group A), HIBD at 24 hours after transplantation (group B), HIBD at 72 hours after transplantation (group C), HIBD at 144 hours after transplantation (group D) E group and HIBDPBS control group (F group), the escape latency of E group and each transplantation group were significantly lower than that of F group (P <0.05), A group was lower than that of B, C and D groups P <0.05), D group was lower than B group, C group (P <0.05). Compared with the F group (P <0.05), the D group was lower than the E group (P <0.05), but higher than the B group And group C (P <0.05). Conclusion Transplantation of rMSCs can significantly improve the spatial learning and memory function in HIBD rats, and transplantation at 2h after HIBD is better than other time points.