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本文报道一种新型的乙型肝炎抗体膜片。测试时,仅需将25μl被检血清滴注于该膜上,经孵育与洗涤即可制得抗原与抗体复合物膜。用复合物膜组装成免疫电极,测定血清样品中乙型肝炎表面抗原含量。电极的线性范围是20～320ng/ml,方法的日间相对标准偏差为10.78％(n=4,m=5),对血清中可能存在的一些其它抗原具有较好的选择性。105例临床血清样本,本法与酶联免疫吸附分析法测定结果符合率为86％。乙肝抗体膜片系一次性使用,4℃可保存半年以上。
This article reports a new type of hepatitis B antibody patch. Test, just 25μl seized serum on the membrane, after incubation and washing can be obtained antigen and antibody complex membrane. The composite membrane was assembled into an immunodetector to measure the level of hepatitis B surface antigen in serum samples. The linear range of the electrode ranged from 20 to 320 ng / ml. The method has a daytime relative standard deviation of 10.78% (n = 4, m = 5) and good selectivity for some other antigens that may exist in the serum. 105 cases of clinical serum samples, this method and enzyme-linked immunosorbent assay results in line with the rate of 86%. Hepatitis B antibody membrane disposable, 4 ℃ can be stored for more than six months.