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目的：揭示正常肝细胞和肝癌细胞对表皮生长因子（ｅｐｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ， ＥＧＦ）不同的应答能力，以及ＥＧＦ对两种不同细胞株内ｒａｓ基因表达的影响。方法：运用Ｗｅｓｔｅｒｎ免疫印迹技术对正常肝细胞株Ｌ－０２和肝癌细胞株Ｈｅｐ－Ｇ２中ｐ２１~(ｒａｓ)的表达进行比较分析。结果：在两种细胞株Ｌ-０２和Ｈｅｐ－Ｇ２中部检测到ｐ２１~(ｒａｓ)的表达。Ｈｅｐ－Ｇ２细胞的ｐ２１~(ｒａｓ)表达量在ＥＧＦ刺激前明显高于Ｌ－０２细胞，经ＥＧＦ刺激２周后，ｐ２１~（ｒａｓ）的表达量持续攀升，表达曲线陡峭。而Ｌ－０２在ＥＧＦ刺激下上升得比较缓慢，到第４周以后基本维持恒定的表达量。结论：正常肝细胞和肝癌细胞内ｒａｓ基因的表达都受ＥＣＦ的影响，但二者对ＥＧＦ的应答能力明显不同。肝癌细胞内ｒａｓ基因的高表达可能与细胞内缺乏有效的反馈机制有关，而ｒａｓ基因的表达持续升高可能是导致细胞增殖失控的一个重要原因。
OBJECTIVE: To reveal the ability of normal hepatocytes and liver cancer cells to respond to epidermal growth factor (EGF) and the effects of EGF on ras gene expression in two different cell lines. Methods: Western blot analysis was used to compare the expression of p21 ras in normal liver cell line L-02 and hepatoma cell line Hep-G2. RESULTS: The expression of p21 ras was detected in the middle of two cell lines L-02 and Hep-G2. The expression of p21 ras in Hep-G2 cells was significantly higher than that in L-02 cells before EGF stimulation. After 2 weeks of EGF stimulation, the expression of p21 ras continued to increase, and the expression curve was steep. L-02 increased slowly under EGF stimulation and remained constant after 4 weeks. Conclusion: The expression of ras gene in normal liver cells and hepatoma cells is affected by ECF, but their response to EGF is obviously different. The high expression of ras gene in hepatocellular carcinoma cells may be related to the lack of effective feedback mechanism in cells, and the continuous increase of ras gene expression may be an important cause of uncontrolled cell proliferation.