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为了探讨小麦HMW-GS和LMW-GS对新疆拉面及蛋白质品质的影响,以195份新疆小麦品种(系)为供试材料,测定其蛋白质品质性状,评价其新疆拉面加工品质,筛选出HMW-GS、LMW-GS存在单亚基等位变异的材料,进行成对数据的t测验。结果表明,就不同位点等位变异对新疆拉面加工品质的贡献大小来讲,Glu-A1位点上,1≥2*>Null;Glu-B1位点上,17+18≥7+9≥7+8≥20≥6+8;Glu-D1位点上,5+10>2≥2+12;Glu-A3位点上,gluA3c≥gluA3a>gluA3d;Glu-B3位点上,gluB3b≥gluB3a>gluB3d>gluB3c≥gluB3g>gluB3j。不同亚基造成新疆拉面加工品质改善的蛋白质机理不同,其中1、2*、7+9、17+18、5+10、gluA3a、gluB3a、gluB3b、gluB3d和gluB3g显著提高了蛋白质的质量;7+8亚基同时显著提高了蛋白质的数量和质量。对于单个亚基而言,Null、7+8、2、gluB3d和gluB3g对籽粒和面粉蛋白质含量,Null、1、2、gluA3d和gluB3d对湿面筋含量,1、2*、7+8、7+9、5+10、gluB3a、gluB3b、gluB3d和gluB3g对Zeleny沉淀值,1、2*、7+8、17+18、5+10、gluB3a和gluB3b对峰值时间,1、7+8、gluA3a、gluB3c、gluB3b、gluB3d和gluB3g对峰值高度,1、2*、7+8、5+10、gluB3a、gluB3b、gluB3d和gluB3g对峰值宽度,1、2*、7+8、17+18、5+10、gluB3a、gluB3b、gluB3d和gluB3g对8min宽度,1、2*、7+8、7+9、17+18、5+10、gluB3a、gluB3b和gluB3g对8min面积均有显著提高或增加的作用。
In order to investigate the effect of wheat HMW-GS and LMW-GS on ramen and protein quality in Xinjiang, 195 Xinjiang wheat cultivars (lines) were used as test materials to determine their protein quality traits, evaluate the processing quality of Xinjiang ramen and select HMW- GS, LMW-GS exist single-subunit allelic variation of the material, the paired data t test. The results showed that, in terms of the contribution of allelic variation at different loci to the processing quality of Xinjiang ramen, 1 ≧ 2 *> Null at Glu-A1 locus, 17 + 18 ≧ 7 + 9 at Glu-B1 locus 7 + 8 ≧ 20 ≧ 6 + 8; Glu-D1, 5 + 10> 2 ≧ 2 + 12; gluA3c ≧ gluA3a> gluA3d at Glu-A3; gluB3b ≧ gluB3a at Glu- > gluB3d> gluB3c> gluB3g> gluB3j. Different subunits caused different processing mechanism of protein in Xinjiang Ramen, in which the protein quality was significantly improved by 1,2 *, 7 + 9, 17 + 18, 5 + 10, gluA3a, gluB3a, gluB3b, gluB3d and gluB3g; 8 subunit at the same time significantly improve the quantity and quality of the protein. Null, 7 + 8,2, gluB3d and gluB3g had no effect on the protein content of grain and flour, the content of Null, 1,2, gluA3d and gluB3d on wet gluten content, 1,2 *, 7 + 8,7 + Zeleny sedimentation value, 1, 2 *, 7 + 8, 17 + 18, 5 + 10, gluB3a and gluB3b peak times, 1, 7 + 8, gluA3a, gluA3a, The peak width, 1,2 *, 7 + 8, 17 + 18, 5 + 10, and peak width of gluB3c, gluB3b, gluB3d and gluB3g for peak height, 10, gluB3a, gluB3b, gluB3d and gluB3g on the 8min width, 1,2 *, 7 +8,7 +9,17 +18,5 +10, gluB3a, gluB3b and gluB3g on the 8min area were significantly increased or increased role .