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本研究旨在探索不同基因区的使用对HIV-1B′亚型毒株系统进化分析结果的影响。首先利用既往研究中已发表的共计47条来自泰国,缅甸和中国多个地区不同传播途径的B′毒株近全长基因组序列,将其按基因区分为不同的数据集(gag,pol,vif,vpr,vpu,env,nef),并分别进行系统进化分析研究。比较不同基因区系统进化分析的结果发现,B′亚型毒株pol基因在分析的基因区中,具有最低的复杂度和进化速率,可以较好的区分B′TH和B′YN毒株,重复近全长基因组序列的分析结果;尽管env基因则具有最高的复杂度和进化速率,但无法获得类似结果。本研究比较了不同基因区对HIV-1B′亚型毒株系统进化分析结果的影响,对进一步开展HIV分子流行病学调查,分析我国B′毒株在我国的传播奠定了基础。
This study aimed to explore the influence of different gene regions on the phylogenetic analysis of HIV-1B subtype strains. First, we used the published sequences of 47 near-full-length genomic sequences of B ’strains from different transmission routes in Thailand, Myanmar and China, and divided them into different data sets (gag, pol, vif , vpr, vpu, env, nef), respectively, and phylogenetic analysis. Comparing the phylogenetic analysis of different gene regions, we found that the pol gene of B ’subtype has the lowest complexity and evolution rate in the analyzed genomic regions, which can distinguish B’TH and B’YN strains well, Repeating the analysis of near-full-length genomic sequences; similar results were not obtained although the env gene had the highest complexity and evolutionary rate. This study compared the effects of different genomic regions on the phylogenetic analysis of HIV-1B subtype strains and laid the foundation for further investigation of HIV molecular epidemiology and analysis of the spread of B ’strains in China.