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旨在生理生化水平上探索柳枝稷苗期慢发育的机理。采用栽培品种‘Alamo’的2个单株(编号为Ma和Mg)收获的种子为材料,在播种后16、24和32d分别对生长发育快(简称快发育苗)和生长发育慢(简称慢发育苗)的2类幼苗进行生长相关的生理指标测定和比较分析。结果表明:慢发育苗地上部分和地下部分鲜质量、干质量显著小于快发育苗,且根长较短,根数较少,但两者根冠比、根系活力无显著差异;慢发育苗与快发育苗相比还原型谷胱甘肽(GSH)质量分数在前期较低,但两者叶绿素、可溶性糖、淀粉、纤维素、可溶性蛋白、全氮和游离氨基酸质量分数总体上无显著差异;慢发育苗脱落酸(ABA)质量分数总体上比快发育苗高,赤霉素(GA)质量分数24d时较低,IAA/ZR比值32d时较低。Ma和Mg同种类型幼苗根长、16d时GSH质量分数、32d时IAA/ZR比值存在显著差异,根数、ABA质量分数和24d时GA质量分数无显著差异。柳枝稷苗期根系不发达,前期GSH质量分数较低,内源激素ABA质量分数较高,中期GA质量分数和后期IAA/ZR比值较低对柳枝稷苗期慢发育的形成有重要影响。
Aims To explore the physiological and biochemical level of switchgrass at the seedling stage of the mechanism of slow development. Seeds harvested from two cultivars ’Alamo’ (Ma and Mg) were used as materials. After 16, 24 and 32 days after sowing, the growth and development (referred to as fast growing seedling) and growth and development slow (referred to as slow Developmental seedlings) 2 kinds of seedlings growth-related physiological indicators were measured and comparative analysis. The results showed that the fresh weight and dry weight of the above-ground part and the underground part of the slow-growing seedling were significantly less than those of the fast-growing seedling, and the root length was short and the number of roots was small, but there was no significant difference between the two. The content of GSH in fast - growing seedlings was lower in early stage, but there was no significant difference in mass fraction of chlorophyll, soluble sugar, starch, cellulose, soluble protein, total nitrogen and free amino acids. The abscisic acid (ABA) content of the slow-growing seedling was generally higher than that of the fast growing seedling, the content of gibberellin (GA) was lower at 24d, and the IAA / ZR ratio was lower at 32d. There were significant differences in root length, GSH mass fraction at 16d, and IAA / ZR ratio at 32d between Ma and Mg isoforms. There was no significant difference in root mass, ABA mass fraction and GA mass fraction at 24d. The root system of switchgrass was underdeveloped at the early stage, with low GSH content, high ABA content, low mid-term GA and low IAA / ZR ratio.