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从大连湾地区健康的刺参养殖池塘沉积物中分离出一株优势菌株B4,通过菌落特征、生理生化特性和16SrDNA同源性分析,确定菌株B4为芽孢杆菌属。该菌株在温度20~35℃,pH 6~9,盐度3~7.5下生长良好。采用108cfu/mL密度的B4菌液投喂仿刺参,投喂后第0、7、14、21d和第28d测定仿刺参体腔液中酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶、溶菌酶和酚氧化酶的活性。试验结果表明,第7d时,试验组仿刺参腔液中碱性磷酸酶、超氧化物歧化酶和酚氧化酶的活性显著高于对照组(P<0.05);第21d时,试验组仿刺参腔液中溶菌酶的活性显著高于对照组;第28d时,酸性磷酸酶的活性与对照组具有极显著差异(P<0.01),说明B4可提高仿刺参体腔液中酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶、溶菌酶和酚氧化酶5种免疫酶的活性,对仿刺参有一定的免疫刺激作用。
A dominant strain, B4, was isolated from healthy sea cucumber aquaculture pond sediments in Dalian Bay. Bacillus was identified as B4 by colony characteristics, physiological and biochemical characteristics and 16S rDNA homology analysis. The strain grew well at the temperature of 20-35 ° C, the pH of 6-9 and the salinity of 3-7.5. The sea cucumber was fed with B4 solution of 108 cfu / mL density. After acid phosphatase, alkaline phosphatase, superoxide dismutase , Lysozyme and phenoloxidase activity. The results showed that the activity of alkaline phosphatase, superoxide dismutase and phenoloxidase in the sea cucumbers of experimental group was significantly higher than that of the control group on the 7th day (P <0.05). On the 21st day, The activity of lysozyme in acanthopanax senticosus fluid was significantly higher than that in the control group. On the 28th day, the activity of acid phosphatase was significantly different from that in the control group (P <0.01), indicating that B4 could increase the acid phosphatase , Alkaline phosphatase, superoxide dismutase, lysozyme and phenoloxidase activity of 5 kinds of immune enzymes, imitation sea cucumber have a certain immune stimulating effect.