目的探究葛根素联合雌二醇干预对去卵巢大鼠骨质疏松以及认知功能障碍的改善效果。方法选取SD雌性大鼠80只,随机等分成5组。其中4组切除双侧卵巢,根据模型制备后不同处理方法分为:模型对照组、葛根素组、雌二醇组以及葛根素联合雌二醇组各16只,剩余16只作为假手术对照组。处理30 d后,比较各组大鼠的学习记忆能力、骨质疏松程度以及血清中钙、磷和碱性磷酸酶的含量。结果随着训练天数延长,各组逃避潜伏期均逐渐缩短,至给药前第1天时,葛根素组联合雌二醇组与单用雌二醇相比,差异有统计学意义(P<0.05)。与假手术对照组相比,模型组跨越平台次数以及在平台象限停留时间明显减少,差异有统计学意义(P<0.05),模型组中上述指标由高到低依次为葛根素联合雌二醇组、雌二醇组、葛根素组和模型对照组,其中联合用药与单用雌二醇相比,差异无统计学意义(P>0.05);模型对照组的骨组织呈骨质疏松病理改变,用药组得到明显改善,其中葛根素联合雌二醇组改善最明显。联合用药与单用雌二醇相比,血清钙、磷以及碱性磷酸酶水平差异无统计学意义(P>0.05)。结论对于去卵巢大鼠认知功能的改善,葛根素联合雌二醇效果明显优于单独用药,且对骨质疏松的治疗效果与单用大剂量雌二醇相近。 Objective To investigate the effect of puerarin and estradiol on osteoporosis and cognitive dysfunction in ovariectomized rats. Methods Eighty female SD rats were randomly divided into five groups. Four groups of ovariectomized were divided into four groups according to different treatment methods: model control group, puerarin group, estradiol group and puerarin combined with estradiol group, and the remaining 16 as sham operation control group . After 30 days of treatment, the learning and memory abilities, the degree of osteoporosis and the levels of serum calcium, phosphorus and alkaline phosphatase in each group were compared. Results With the prolongation of training days, the escape latency of each group was gradually shortened. There was a significant difference between the puerarin group and the estradiol group on the first day before the administration (P <0.05) . Compared with the sham-operation control group, the number of platform crossing the platform and the dwell time in the platform quadrant decreased significantly (P <0.05), and in the model group from high to low were puerarin and estradiol Estradiol group, puerarin group and model control group. There was no significant difference between the combination therapy and the single administration of estradiol (P> 0.05). The bone tissue of the model control group showed pathological changes of osteoporosis , Medication group was significantly improved, in which puerarin and estradiol group to improve the most obvious. Compared with single administration of estradiol, there was no significant difference in serum calcium, phosphorus and alkaline phosphatase (P> 0.05). Conclusion The improvement of cognitive function in ovariectomized rats shows that puerarin combined with estradiol is superior to that of puerarin alone and its therapeutic effect on osteoporosis is similar to that of high-dose estradiol alone.