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目的研究氟暴露大鼠肝脏生化指标的变化,探讨氟暴露对肝脏功能的影响。方法 96只SD大鼠,分为6组,每组16只,雌雄各半,隔天灌胃给予不同剂量氟化钠溶液(0、2、4、8、16、32 mg/kg)。染毒期间,隔天称量大鼠体重;3个月后,处死大鼠,计算肝脏系数并测定肝功能生化指标中血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活力以及总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)浓度。结果 16和32 mg/kgNaF组雄性大鼠肝脏系数分别为2.660±0.091和2.700±0.101,雌性大鼠分别为2.722±0.225和2.736±0.216,均高于对照组的2.526±0.086和2.409±0.085,差异均有统计学意义(均P<0.05);AST活力降低,差异有统计学意义(P<0.01)。结论高剂量氟暴露可使大鼠AST活力降低,可将AST作为高剂量氟离子暴露引起肝脏损伤的临床观察指标。
Objective To investigate the changes of liver biochemical parameters in rats exposed to fluorine and to explore the effect of fluoride exposure on liver function. Methods 96 Sprague-Dawley rats were randomly divided into 6 groups with 16 rats in each group. The rats in each group were given half a dose of sodium fluoride solution (0,2,4,8,16,32 mg / kg) by intragastric administration. Rats were weighed on the next day during the exposure period. After 3 months, rats were sacrificed and liver coefficients were calculated. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity and Total protein (TP), albumin (ALB), globulin (GLB) concentrations. Results The liver coefficients of male rats with 16 and 32 mg / kgNaF were 2.660 ± 0.091 and 2.700 ± 0.101, respectively, and those of female rats were 2.722 ± 0.225 and 2.736 ± 0.216, respectively, which were higher than those of control group (2.526 ± 0.086 and 2.409 ± 0.085, (P <0.05). The activity of AST decreased, the difference was statistically significant (P <0.01). Conclusion High-dose fluoride exposure can reduce AST activity in rats, and AST can be used as a clinical observation indicator of liver damage caused by high-dose fluoride exposure.