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目的 建立一种能动态显示活体内肿瘤细胞生长、新生血管形成及靶基因表达水平等的新型动物模型。方法 以海洋水母中分离到的绿色荧光蛋白 (GFP)作为报告基因 ,将 1× 10 3～ 1× 10 4 持续、稳定、高水平表达GFP的肿瘤细胞移植到鼠皮窗内 ;或以 1× 10 8～ 1× 10 9GFP腺病毒感染皮窗肿瘤 ;或将转导了热诱导型表达质粒的肿瘤细胞移植到鼠皮窗内 ,隔天用荧光显微镜进行观察。结果 移植到鼠皮窗内的肿瘤细胞 3～ 7d时可见肿瘤细胞围绕大血管生长及大血管出芽形成新生血管 ,腺病毒的感染效率及靶基因的表达水平可通过GFP表达确定。结论 新型肿瘤皮窗模型可显示肿瘤细胞生长及新生血管形成过程 ,病毒等对肿瘤细胞的感染效率以及靶基因在肿瘤内的表达水平等
Objective To establish a novel animal model that can dynamically display tumor cell growth, neovascularization and target gene expression in vivo. Methods The green fluorescent protein (GFP) isolated from marine jellyfish was used as a reporter gene to transplant 1×10 3 - 1×10 4 tumor cells with persistent, stable and high level of GFP expression into rat skin window; or with 1× 10 8 to 1×10 9 GFP adenoviruses infected the window tumors; or tumor cells transduced with heat-inducible expression plasmids were transplanted into the rat skin window and observed by fluorescence microscopy on alternate days. Results Tumor cells transplanted into rat skin window showed tumor cells around the large blood vessels and budding of large blood vessels to form new blood vessels at 3-7 d. The infection efficiency of adenovirus and the expression level of target genes could be confirmed by GFP expression. Conclusion The novel tumor window model can show the growth and neovascularization of tumor cells, the infection efficiency of virus and other tumor cells, and the expression level of target genes in tumors.