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１９９０年 ６－９月，从青岛上马镇对虾养殖场患败血症的中国对虾血淋巴内分离到副溶血弧菌２５－Ｃ菌株，利用平板玻璃纸覆盖技术，获得２５－Ｃ菌株的胞外产物（ＥＣＰ），进行成分分析和致病性研究。结果表明，副溶血弧菌２５－Ｃ菌株的胞外产物具有多种酶活性和溶血活性，是病原菌感染中国对虾的主要因素，单独注射感染即可导致中国对虾的严重发病至死亡，ＥＣＰ蛋白可以破坏中国对虾血清的体外杀菌活力，提高２５－Ｃ菌株对中国对虾的致病能力。聚丙烯酰胺凝胶电泳分析结果表明，ＥＣＰ蛋白中含有２３条蛋白带。对具有溶血活性的蛋白组分进行热稳定性测试后，认为有两种溶血因子。经感染分析，发现其中具有热稳定性溶血活性和蛋白酶活性的两组蛋白对中国对虾具有明显的致病作用，可能是决定细菌毒力的主要因素。
From June to September 1990, Vibrio parahaemolyticus strain 25-C was isolated from hemolymph of Penaeus chinensis infected by septicemia in shrimp farms of Shangma Town, Qingdao City. Extracellular products of 25-C strain (ECP ) For component analysis and pathogenicity studies. The results showed that the extracellular products of Vibrio parahaemolyticus 25-C had various enzyme activities and hemolytic activities, which were the main factors of pathogenic bacteria infection of P. shrimp. Single infection could lead to the serious death of P. shrimp and ECP protein could Destroy the in vitro bactericidal activity of Chinese shrimp serum and improve the pathogenicity of 25-C strain on Chinese shrimp. Polyacrylamide gel electrophoresis analysis showed that ECP protein contains 23 protein bands. After the thermostability test is performed on the hemolytic protein component, it is considered that there are two kinds of hemolysis factors. The infection analysis showed that the two groups of proteins with thermostable hemolytic activity and protease activity had obvious pathogenicity on P. shrimp and may be the main factor to determine the bacterial virulence.