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以β环糊精(β-CD)功能化金纳米棒为光学探针,基于β-CD对芒果苷、白杨素及瑞香素多酚化合物的包合作用,建立了一种检测疏水性多酚化合物的新方法。多酚化合物在体积分数10%的甲醇水溶液(pH 6.0)介质中,与β-CD功能化金纳米棒作用10 min,β-CD的包合作用使其富集到金纳米棒表面,从而引起金纳米棒表面等离子共振吸收光谱发生变化;以质谱等技术证明了β-CD对多酚化合物具有包合作用。随着多酚化合物浓度的增加,金纳米棒探针在约为700 nm处的等离子共振吸收峰随之线性下降,对芒果苷、白杨素和瑞香素的检出限分别为9.0×10-9,5.0×10-8,4.0×10-8mol/L,建立了一种可在水溶液中富集与检测疏水性多酚化合物的新方法。
Based on β-cyclodextrin (β-CD) functionalized gold nanorods as optical probe, based on the inclusion of mangiferin, chrysin and daphnetin polyphenols, β-CD was used to detect hydrophobic polyphenols New method of compound. The polyphenolic compounds were mixed with β-CD functionalized gold nanorods in 10% methanol aqueous solution (pH 6.0) for 10 min. The inclusion of β-CD enriched the surface of gold nanorods, The surface plasmon resonance absorption spectra of gold nanorods changed. The inclusion complexes of β-CD with polyphenols were proved by mass spectrometry. With the increase of the concentration of polyphenol compounds, the absorption peak of plasma resonance of gold nanorod probe decreased linearly at about 700 nm, and the detection limits of mangiferin, chrysin, daphnetin were 9.0 × 10-9 , 5.0 × 10-8,4.0 × 10-8mol / L, a new method for enrichment and detection of hydrophobic polyphenol compounds in aqueous solution was established.