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目的 研究低强度微波辐射对兔眼晶状体上皮细胞细胞间隙连接通讯功能的影响及其机理。方法 健康家兔接受 3h 5mW/cm2 或 10mW/cm2 微波辐射。取兔双眼晶体前囊膜 ,用 5 ,6 羟基荧光素乙酸乙酰盐染色作激光共聚焦显微镜晶体上皮细胞荧光淬灭及恢复情况测定。用免疫荧光法标记Cx4 3作定位检测。结果 接受 5mW /cm2 或 10mW /cm2 微波辐射后兔眼晶体上皮细胞荧光恢复速度明显减慢 ,GJIC功能破坏。Cx4 3荧光标记定位结果显示 ,5mW/cm2 实验组Cx4 3阳性膜表达减少 ;10mW/cm2 实验组出现Cx4 3的细胞外聚集 ,胞浆和细胞核内位移。结论 5mW/cm2 和 10mW/cm2 低强度微波通过破坏Cx4 3的正常功能定位而抑制晶体上皮细胞间GJIC功能 ,破坏晶体内稳态 ,促使晶体上皮细胞发生凋亡 ,诱发白内障。
Objective To study the effect and mechanism of low intensity microwave radiation on the cell gap junctional intercellular communication of lens epithelial cells in rabbits. Methods Healthy rabbits received 3h 5mW / cm2 or 10mW / cm2 microwave radiation. Rabbit binocular lens anterior capsule was taken and the fluorescence quenching and recovery of crystal epithelial cells were observed by 5, 6 hydroxy fluorescein acetate acetate staining. Cx4 3 was labeled by immunofluorescence for localization. Results After 5 mW / cm2 or 10 mW / cm2 microwave irradiation, the recovery of fluorescence in the lens epithelial cells of rabbits was significantly slowed down and GJIC function was impaired. The results of Cx4 3 fluorescent labeling showed that the expression of Cx4 3 positive membrane in 5 mW / cm2 experimental group was decreased. The extracellular aggregation of Cx4 3 and the intracellular nuclear shift were observed in 10 mW / cm2 experimental group. Conclusion Low intensity microwaves of 5mW / cm2 and 10mW / cm2 can inhibit the function of GJIC and disrupt crystal homeostasis by disrupting the normal function of Cx4 3, leading to the apoptosis of lens epithelial cells and inducing cataract.