论文部分内容阅读
目的研究砷暴露对不同发育阶段小鼠仔鼠海马N-甲基-D-天冬氨酸受体(NMDAR)水平的影响,探讨砷暴露引起学习记忆能力损伤的可能机制。方法建立不同发育阶段小鼠仔鼠砷暴露模型,砷暴露浓度分别为0、15、30和60 mg/L,记录体重,分别于仔鼠出生后(postnatal day,PND)10、20、40 d取脑组织并分离海马,采用RT-PCR检测NMDAR亚单位NR1、NR2A和NR2B m RNA水平。结果 60 mg/L砷暴露组仔鼠PND20的体重低于对照组和15 mg/L组,30、60 mg/L砷暴露组仔鼠PND40的体重低于对照组,差异均有统计学意义(P<0.05)。各浓度砷暴露组仔鼠PND20的海马NR1和NR2B m RNA水平均低于对照组,60 mg/L砷暴露组仔鼠PND20的海马NR2A水平低于对照组;60 mg/L砷暴露组仔鼠PND40的海马NR1、NR2A和NR2B m RNA水平低于对照组,差异有统计学意义(P<0.05)。结论砷暴露可使仔鼠海马NMDAR亚单位的水平降低,进而可能影响仔鼠的学习记忆能力。
Objective To investigate the effect of arsenic exposure on the level of NMDAR in hippocampus of offspring mice in different developmental stages and to explore the possible mechanism of arsenic exposure in learning and memory impairment. Methods The arsenic exposure model of offspring mouse was established at different developmental stages. The arsenic exposure concentrations were 0, 15, 30 and 60 mg / L, respectively. Body weight was recorded at postnatal day (PND) The brain tissues were removed and the hippocampus was separated. The NR1, NR2A and NR2B m RNA levels of NMDAR subunits were detected by RT-PCR. Results The body weight of PND20 in the arsenic-exposed group was lower than that of the control group and 15 mg / L group. The body weight of PND40 in the 30,60 mg / L arsenic-exposed group was lower than that in the control group P <0.05). The levels of NR1 and NR2B m RNA in hippocampus of PND20 in arsenic exposure group were lower than that in control group, while the NR2A level in PND20 of 60 mg / L arsenic exposure group was lower than that of control group. The levels of NR1, NR2A and NR2B m RNA in PND40 hippocampus were lower than those in control group, with statistical significance (P <0.05). Conclusion Arsenic exposure may decrease the level of NMDAR subunits in the hippocampus of the offspring, which may affect the learning and memory abilities of the offspring.