目的　观察雌激素受体阻断剂tamoxifen对大鼠垂体前叶细胞增殖的影响。方法　应用大鼠垂体前叶细胞原代培养和3 H TdR参入法检测细胞增殖 ,用电镜观察细胞形态学的改变。结果　tamoxifen能抑制大鼠垂体前叶细胞增殖 ,0 1μmol·L-1tamoxifen的抑制作用可被雌激素反转。 1μmol·L-1tamoxifen作用 4 8h ,细胞出现典型的凋亡改变。结论　tamoxifen抑制大鼠垂体前叶细胞增殖 ,并诱导细胞发生凋亡 Objective To observe the effect of estrogen receptor blocker tamoxifen on the proliferation of anterior pituitary cells in rats. Methods The primary culture of rat anterior pituitary cells and the 3 H TdR incorporation method were used to detect the cell proliferation. The morphological changes of the cells were observed with electron microscope. Results tamoxifen can inhibit rat anterior pituitary cell proliferation, 0 1μmol·L-1tamoxifen inhibition can be reversed by estrogen. The apoptosis of cells was observed after 1μmol·L-1tamoxifen treatment for 48h. Conclusion tamoxifen inhibits the proliferation of rat anterior pituitary cells and induces cell apoptosis