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探索一种无创性动态测定血糖的原理和建立实验方法。使用共价键固定法 ,制作葡萄糖氧化酶传感器 ,选择传感器最佳制作方法和稳定的工作条件。设立超声前、后组 ,注射葡萄糖前、后及高频、低频超声对照组。在 10只日本大耳兔的皮肤表面 ,用高频或低频超声波处理皮肤 5 m in后 ,用葡萄糖氧化酶传感器测定其微电流信号的变化。采用葡萄糖氧化酶传感器和微电流检测仪 ,可观察到经超声波处理后 ,兔皮肤表面 ,微电流信号明显增高变化(P<0 .0 1)和在注射葡萄糖后的进一步增高变化 (P<0 .0 1)。兔皮肤表面经超声波处理后 ,对葡萄糖的通透性明显增高 ,经葡萄糖氧化酶传感器转化为微电流信号的变化 ,与兔体内的葡萄糖水平呈正相关。在活体动物皮肤表面 ,用葡萄糖氧化酶传感器测定血糖是可行的
To explore a noninvasive dynamic measurement of blood sugar principle and establishment of experimental methods. The use of covalent immobilization method, the production of glucose oxidase sensor, the choice of sensors for the best production methods and stable working conditions. Before and after the establishment of ultrasound group, before and after glucose injection, and high-frequency, low-frequency ultrasound control group. After the skin of 10 Japanese big-eared rabbits was treated with high-frequency or low-frequency ultrasound for 5 minutes, the change of the micro-current signal was measured using a glucose oxidase sensor. Using glucose oxidase sensor and micro-current detector, the ultrastructure of rabbit skin surface and micro-current signal were significantly increased (P <0. 01) and further increased after glucose injection (P <0 .0 1). Rabbit skin surface after ultrasonic treatment, the permeability of glucose was significantly increased by the glucose oxidase sensor into micro-current signal changes, and the rabbit’s glucose level was positively correlated. On the surface of living animal skin, it is feasible to measure blood glucose with a glucose oxidase sensor