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目的 探讨FBXO22基因在乳腺癌血管形成中的作用及分子生物学机制.方法 使用FBXO22-SiRNA转染人乳腺癌MDA-MB-231和BT-549细胞,体外血管形成实验观察2种乳腺癌细胞中干扰FBXO22后使人脐静脉内皮细胞(human umbilical vein endothelial cells, HUVECs)血管形成数量的变化;Western blot检测FBXO22-SiRNA对2种乳腺癌细胞中FBXO22、缺氧诱导因子-1α(hypoxia inducible factor - 1,HIF - 1α)、血管内皮生长因子(vascular endothelial growth factor, VEGF)蛋白表达的影响;Real time - PCR检测干扰FBXO22对2种乳腺癌细胞中HIF - 1α和VEGF的mRNA水平的影响.结果 在MDA - MB -231和BT - 549细胞中干扰FBXO22后分为FBXO22 - Ctrl组和FBXO22 - si组.与FBXO22 - Ctrl组相比,MDA -MB-231和BT - 549细胞FBXO22 -si组血管形成数目分别增加了107. 1％和181. 8％;FBXO22蛋白表达量分别减少了55. 5％和51. 9％;HIF-1α蛋白表达量分别上升了39. 3％和68. 4％;VEGF蛋白表达量分别上升了40. 7％和21, 7％;HIF - 1α和VEGF的mRNA水平无明显变化.结论 在乳腺癌中FBXO22作为抑癌基因对血管形成发挥着重要作用,干扰FBXO22后能够通过HIF-1α通路导致VEGF蛋白表达量提高并促进血管形成.“,”Objective To investigate the role effects of FBXO22 gene in the angiogenesis of breast cancer and its molecular biological mechanism. Methods FBXO22 - siRNA was transfected into human breast cancer MDA - MB -231 and BT -549 cells. Then, the number of vessels developed by human umbilical vein endothelial cells (HUVECs) that were affected by the two types of cancer cells under the interference of FBXO22 - siRNA was observed by in vitro angiogenesis experiment. The effects of FBXO22 - siRNA in MDA - MB -231 and BT - 549 cells on FBXO22, hypoxia inducible factor - 1α (HIF - 1α) and vascular endothelial growth factor (VEGF) were detected by Western blotting. The levels of HIF - 1α and VEGF mRNA after FBXO22 interference were examined by real time - PCR. Results After transfection with FBXO22 - Ctrl RNA and FBXO22- siRNA into MDA-MB-231 and BT - 549 cells, the cells were divided into a FBXO22 - Ctrl group and an FBXO22 - si group. Compared with the FBXO22 - Ctrl group, the FBXO22 - si group showed increases in the number of vessels newly formed in MDA - MB -231 and BT -549 cells by 107. 1％ and 181. 8％ respectively, decreases in the levels of FBXO22 by 55. 5％ and 51. 9％, increases in the levels of both HIF - 1 protein by 39. 3％ and 68. 4％ and VEGF protein by 40. 7％ and 21. 7％. There were no significant differences in mRNA level of HIF - 1α and VEGF between the two groups. Conclusions FBXO22 plays an important role in angiogenesis as a tumor suppressor gene in breast cancer. After FBXO22 interference, it can increase the expression of VEGF at the protein level and promote angiogenesis through the HIF - 1α pathway.