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将组织块置于明胶海绵上,摇摆器官培养,4～6周后成人食管粘膜仍保持良好的结构形态,具有活跃生长的上皮细胞和良好的腺体结构;将组织块贴壁,静置外植块—细胞培养,成人食管粘膜和胎儿食管组织块可存活2～3个月,提供大量生长旺盛的上皮细胞。成人食管癌旁粘膜培养利于观察细胞癌前改变,可能较易于诱发癌变;胎儿食管具有正常背景,利于研究细胞癌变的全过程.二者配合可组成研究人食管上皮癌变的完整体外实验系统。
The tissue was placed on a gelatin sponge, swing organ culture, 4 to 6 weeks after the adult esophageal mucosa still maintain a good structural morphology, with active growth of epithelial cells and good glandular structure; Plant-cell culture, adult esophageal mucosa and fetal esophageal tissue block can survive 2 to 3 months, providing a large number of vigorous growth of epithelial cells. Paracancerous mucosal culture of adult esophageal cancer is conducive to observation of precancerous changes, may be more likely to induce cancer; fetal esophagus has a normal background, which will help to study the entire process of cell carcinogenesis. The two combined to form a complete in vitro experimental system of esophageal cancer.