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研究载脂蛋白ＡＩ启动子－７５ｂｐ处Ｇ／Ａ嘌呤置换以及饮酒对凉山彝族及汉族人群血浆载脂蛋白ＡＩ水平 的影响。经现场调查随机抽样７４２名凉山州彝汉健康居民，包括三个人群样本：彝族农民、彝族移民、汉族居 民。根据饮用酒精量将各人群分为四组：不饮酒者、 １～２５ｇ／ｄ饮酒者、 ２６～７５ｇ／ｄ以上饮酒者、 ７５ｇ／ｄ以上饮酒 者。采集清晨空腹血样１０ｍＬ／人。全项血脂和脂蛋白水平测定。从冷冻血凝块中抽提基因组ＤＮＡ，经ＰＣＲ扩 增，进行片段长度多态性分析。结果：随着饮酒量的增加，血浆ＨＤＬＣ和ａｐｏＡＩ水平逐渐升高，在彝族农民 样本和汉民样本中有非常显著的统计学意义。三人群样本间的Ａ等位基因频率相似。Ａ等位基因携带者与ＧＧ 基因型携带者之间的各项血脂水平无显著差异。但在不饮酒者中，Ａ等位基因携带者的ａｐｏＡＩ水平明显高于 ＧＧ基因型携带者；而饮酒者中，Ａ等位基因携带者的ａｐｏＡＩ和ＨＤＬＣ水平明显低于ＧＧ基因型携带者，上述 差异在城镇人群中显示出统计学意义（Ｐ＜０．０５）。不饮酒者中，Ｇ／Ａ置换可以解释１８％的个体间ａｐｏＡＩ水平变 异（Ｆ＝８．９４，Ｐ＜０．０１）。结论；适量饮酒和ａｐｏＡＩ基因－７５处Ｇ／Ａ置换多态性均可使?
To investigate the effects of G-A purine replacement at-75bp at the apolipoprotein AI promoter and alcohol consumption on plasma apolipoprotein AI in Liangshan Yi and Han populations. A total of 742 healthy residents of Yi and Han nationality in Liangshan Prefecture were surveyed by field survey, including three sample populations: Yi peasants, Yi immigrants and Han residents. According to the amount of drinking alcohol, each group is divided into four groups: non-drinkers, 1 ~ 25g / d drinkers, 26 ~ 75g / d drinkers and 75g / d drinkers. Fasting blood samples collected early 10mL / person. Whole blood lipids and lipoprotein levels were measured. Genomic DNA was extracted from frozen blood clot and amplified by PCR for fragment length polymorphism analysis. Results: With the increase of alcohol consumption, plasma HDLC and apoAI levels gradually increased, and there was a significant statistical significance in the Yi peasant sample and Han sample. The A allele frequencies were similar between the three-person samples. There was no significant difference in each lipid level between carriers of A allele and GG genotype. However, in non-drinkers, the level of apoAI in carriers of allele A was significantly higher than those in carriers of genotype GG. In drinkers, the level of apoAI and HDLC in carrier A was significantly lower than that of carriers of GG, The above differences showed statistical significance in urban population (P <0.05). Among non-drinkers, G / A replacement explained 18% of individuals with apoAI level variation (F = 8.94, P <0.01). Conclusion; moderate alcohol consumption and apoAI gene -75 G / A substitution polymorphism can make?