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目的:建立紫外分光光度法测定排石颗粒中总黄酮含量的不确定度评定方法,找出影响不确定度的因素。方法:采用紫外分光光度法测定总黄酮的含量,并依据《测量不确定度评定与表示》(JJF 1059-1999)中有关规定评估其不确定度。结果:扩展不确定度为7.99%,含量测定结果可表示为(9.94±0.79)mg·g~(-1)。结论:紫外测定总黄酮含量的不确定度主要由标准曲线的拟合、对照品与样品的配制和稀释引入。
OBJECTIVE: To establish a UV spectrophotometric method for the determination of the uncertainty of total flavonoids in Paishi granules, and find out the factors affecting the uncertainty. Methods: The content of total flavonoids was determined by UV spectrophotometry, and its uncertainty was evaluated according to the relevant regulations of “Evaluation and Expression of Uncertainty in Measurement” (JJF 1059-1999). Results: The uncertainty of expansion was 7.99%, and the result of determination was (9.94 ± 0.79) mg · g -1. Conclusion: The uncertainty of determination of total flavonoids in UV is mainly caused by the fitting of standard curve and the formulation and dilution of reference substance and sample.