以往制备斯氏肺吸虫水溶性抗原 ( Ps-AWA) ,均为短时间 ( 4～ 5d)一次性浸提 ,残渣即被弃去。然而 ,近年来多数斯氏肺吸虫病流行区的生态环境已经或正在发生变化 ,蟹体内囊蚴的感染密度较以往大大降低 ,这就给本病的实验研究尤其是制备抗原带来一定困难 ,如何有效利用虫体资源 In the past, Ps-AWA was prepared from Pseudomonas aeruginosa, which were all leached in a short time (4 ~ 5 days). The residue was discarded. However, in recent years, the ecological environment of most endemic areas of Asperger’s disease has been or is changing, and the infection density of metacercariae in the crab is much lower than in the past. This poses some difficulties for the experimental study of this disease, How to make effective use of insect resources