据统计,日本1976年死于肝癌的人数剧增,达10万分之9,1987年增加一倍,为10万分之18。其中HBs抗原阳性者为10万分之4,无增加,骤增的病例均非乙型肝炎。非乙型肝硬化、肝癌病例40%有输血史。输血后非甲非乙型肝炎可有肝硬化和肝癌发生。1988年Chiron社一个小组成功开发了用C_(100)-3抗原的抗体(HCVAb)测定方法,此抗原在酵母中发现,并作为HCV感染的特异标志。1989年2～3月检查了东大、山梨医大、信州大、 Statistics show that the number of deaths from liver cancer in Japan increased dramatically in 1976 to 9/10 in 1995 and doubled in 1987 to 18/10 in 18. The HBs antigen-positive patients were 4 out of 10, no increase, no sudden increase of hepatitis B cases. Non-B cirrhosis, liver cancer cases 40% have a history of blood transfusions. Non-A non-B hepatitis after transfusion may have cirrhosis and liver cancer. In 1988, a group of Chiron Society successfully developed the method of determination of antibody to C_ (100) -3 antigen (HCVAb), which is found in yeast and serves as a specific marker of HCV infection. From February to March 1989, we examined Dongda University, Yamanashi University, Shinshu University,