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目的研究糖尿病大鼠肾脏内源性硫化氢(H2S)的两种合成酶胱硫醚-β-合成酶ystathionine-β-synthase CBS)和胱硫醚-γ-裂解酶(cystathionine-γ-lyase CSE)mRNA表达。方法用腹腔注射链脲佐菌素诱发糖尿病模型,大鼠随机分为两组:正常对照组(C组)和糖尿病组(D组)。建模第8周,测定24h尿蛋白、血糖及肌酐清除率[1],应用RT-PCR技术检测大鼠肾皮质CBS、CSE mRNA表。结果糖尿病组大鼠尿蛋白、血糖及肌酐清除率均较正常对照组明显升高(P<0.05),但肾皮质CBS、E mRNA表达较正常对照组无显著性差异(P>0.05)。结论CBS、CSE/H2S系统不能通过两种合成酶NA水平的改变而参与糖尿病肾病中血流动力学紊乱发生,其作用通路中的其他环节改变是否参与糖尿病病的发生发展尚需进一步证实。
OBJECTIVE To study the effects of two kinds of synthetic enzymes such as ystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), two kinds of synthetases of endogenous hydrogen sulfide (H2S) ) mRNA expression. Methods The diabetic model was induced by intraperitoneal injection of streptozotocin. The rats were randomly divided into two groups: normal control group (C group) and diabetic group (D group). At the 8th week of modeling, 24-hour urinary protein, blood glucose and creatinine clearance were measured [1]. The mRNA expressions of CBS and CSE in rat renal cortex were detected by RT-PCR. Results The urinary protein, blood glucose and creatinine clearance in diabetic rats were significantly higher than those in normal controls (P <0.05). However, the expressions of CBS and E mRNA in renal cortex were not significantly different from those of normal controls (P> 0.05). Conclusion CBS and CSE / H2S systems can not participate in the hemodynamic disturbances in diabetic nephropathy by changing the levels of two kinds of synthase NA. It is necessary to confirm whether other pathways involved in the pathogenesis of diabetes are involved in the development of diabetes mellitus.