论文部分内容阅读
研究中药粗提剂影响体外淋巴细胞转化试验时,常因所含杂质多浓度偏高或细胞收集不全,使 ~3H 标记的胸腺嘧啶脱氧核糖苷(~3H-TdR)掺入受阻或细胞丢失而致 cpm 偏低,造成假阴性。加用镜检观察细胞增殖为指标时,它受上述因素干扰较小,可有很高的可信性、重复性,较宽的检出浓度范围,较易检测成功,以及可多人同时评定使其客观性较强等特点,它与~3H—TdR 掺入指标两者互相参比,可获客观确切结果。增强细胞收集时所用负压.延长冲吸时间、镜验收集是否完全等,可防止中药粗提剂增强细胞粘附培养板孔面而致收集不全之弊。
Studies on crude extracts of traditional Chinese medicines affect lymphocyte transformation assays in vitro, often due to high concentrations of impurities or incomplete cell collection, which results in the incorporation of ~3H-labeled thymidine (~3H-TdR) into blocked or lost cells. Caused low cpm, resulting in false negatives. When microscopic observation of cell proliferation is used as an index, it is less affected by the above factors, it can have high credibility, repeatability, a wider range of detected concentrations, easier detection, and can be assessed by many people at the same time. It is characterized by its strong objectivity, and it is mutually comparable with the ~3H-TdR incorporation index, which gives objective and exact results. Enhance the negative pressure used in cell collection. Prolong the time of scavenging and complete the collection of microscope examination to prevent the disadvantages of incomplete collection caused by the crude extract of traditional Chinese medicine enhancing cell adhesion to the plate surface.